TABLE 2.
DNA sequences of the primers used in this study
| Primer name | Nucleotide sequence (5′ to 3′)a |
|---|---|
| vcrx076del.f | CTTATTTGCTCAAAAAGGGCACTTCCACAGCCCATGCGGAATAGGAACTTCAAGAT |
| vcrx076del.r | TCTGAACCGGGCTTTGAGAGGAATGAACCTTAGTTCAGGAACTTCAGAGCGCTT |
| 94del145.for | ATGCCGTGGTAAACTGGAGGTAAAGAGTAAGGGGGTTGATgtgtaggctggagctgcttc |
| 94del145.rev | ACCAACCAAATAATAAGGGGGCCAGCAGGCCCCCTTATTACATATGAATATCCTCCTTA |
| 94vcrx145.for | NNNNNNGAATTCAAGGAGGAATAATAAATGAAACATGTGGTCAATATTCTT |
| 94vcrx145.rev | NNNNNNGAATTCTTATTCGTCTCCAGCTCCAA |
| pAsa4cTrxA.f | GTACGAATTCAAGGAGGAATAATAATTGAGGACCTGCCTGTAT |
| pAsa4cTrxA.r | AGGTGAATTCTCATTCACCCCAACGAGCT |
| ptrxAmut.f | ATGAGGAATGTGGTTAAC |
| ptrxAmut.r | TTATTATTCCTCCTTGAATTCG |
| pAsa4cTraG.f | NNNNNNGAATTCAAGGAGGAATAATAAATGGGTACGTTCACAATCTA |
| pAsa4cTraG.r | NNNNNNGAATTCTTAATGCTTCGAATCTAAGCCT |
| eexC183.f | TGGTTGCCATTGTTCGTGCCGCGCGGTCAGCATATAAAGAG |
| eexC279.r | CTCTTTATATGCTGACCGCGCGGCACGAACAATGGCAACCA |
| eexC280.f | CTAACGTGCTTTATCCGCGTTCTCATAAAGGCGAGAAGAGAG |
| eexC182.r | CTCTCTTCTCGCCTTTATGAGAACGCGGATAAAGCACGTTAG |
| ptrxA2.f | GTCAGAATTCAAGAGGGAGAGCGTGATGAG |
| toxdel-F | GGTCTGCAATTGGGATCATTACTTCATAAAACCTTTTCTCGTGTAGGCTGGAGCTGCTTC |
| toxdel-R | CAACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATGCCATGGTCCATATGAATATCCT |
| Del1-Kan-F | CTGCACGAACACCAGATCTGATATGTGGGAACGCTGCACGAAGATCCCCTCACGCTGCCG |
| Del1-Kan-R | GTGCCTCTAACGCCCCCGAGCAAAAGCCTCAGCCCCAGATCAGAAGAACTCGTCAAGAAG |
| Alt-del2-F | TGCATCTGTGCGACATCCCATTGAATGTCTACCAGGTGATAAGATCCCCTCACGCTGCCG |
| new-Alt-del2-R | CCGAATGTTTTTCCGAAGCCGCAACGCTAACTTCATTGGCTCAGAAGAACTCGTCAAGAA |
| new-del4-F | ACTCTGCTTTTCCCTGATCAAGAAGGTAAGGGAAAGCAACGTCGTGACTGGGAAAACCCT |
| new-del4-R | CTTCTGTCGCTTCTGCGGCCATCTTCATCTGCTTCTCGACACTCCAGCATATTCAGCGAC |
a
EcoRI restriction sites are underlined.