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. 2019 Apr 24;201(10):e00801-18. doi: 10.1128/JB.00801-18

FIG 1.

FIG 1

APS biosynthesis governed by the uxs1-apsH2 gene cluster facilitates biofilm formation of S. meliloti. Biofilm formation of S. meliloti strains, harboring empty vector pWBT and pWBT-pleD-cuxR (A) or empty vector pSRKGm and pSRKGm-uxs1-SMb20463 (B), assessed by crystal violet staining of cell material attached to polystyrene surface. Gene overexpression was induced by adding 0.5 mM IPTG to the medium. Biofilm formation is shown relative to that of the wild type carrying the respective empty vector. Error bars represent the standard deviations from four biological replicates. Congo red (CR)-stained macrocolonies of indicated strains are also shown.