FIG 2.

uxs1, uxe, and apsS are important for APS-facilitated biofilm formation of S. meliloti. (A) Schematic representation of the APS biosynthesis gene cluster uxs1-apsH2 with annotated gene products. (B) Macrocolonies of Rm2011 and deletion strains, harboring either empty vector pWBT or pWBT-pleD-cuxR, grown on MM agar supplemented with CR and 0.5 mM IPTG. (C) Biofilm formation on polystyrene surface of Rm2011 and uppE mutant with indicated APS biosynthesis gene deletions, each harboring either empty vector pWBT or pWBT-pleD-cuxR. Gene overexpression was induced by adding 0.5 mM IPTG to the medium. Biofilm formation is shown relative to that of the wild type carrying the empty vector. Error bars represent the standard deviations from four biological replicates.