TABLE 1.
Carbohydrate fingerprints of S. melilotia
| Genetic background | pleD++ cuxR++b | Mean (range) content (mg/liter) forc: |
||||
|---|---|---|---|---|---|---|
| Glucose | Galactose | Xylose/arabinose | Cellobiose | Gentiobiose | ||
| ΔexoP-Z wgeB | − | 124 (7) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| ΔexoP-Z wgeB | + | 143 (18) | 9 (5) | 54 (1) | 0 (0) | 0 (0) |
| ΔexoP-Z wgeB | + | 317 (195)d | 0 (0)d | 145 (14)d | 0 (0)d | 0 (0)d |
| ΔexoP-Z wgeB Δuxs1-apsH2 | + | 317 (17) | 11 (0) | 0 (0) | 0 (0) | 0 (0) |
| ΔexoB | + | 402 (13) | 54 (10) | 34 (2) | 15 (2) | 8 (2) |
a
S. meliloti Rm2011 mutants lacking the succinoglycan biosynthesis gene cluster (ΔexoP-Z), carrying a plasmid insertion inside the wgeB gene of the galactoglucan biosynthesis gene cluster (wgeB), containing a deletion of the exoB gene, and/or lacking the APS biosynthesis gene cluster (Δuxs1-apsH2) were used to determine carbohydrate fingerprints of culture supernatants.
b
Strains harbored either empty vector pWBT (−) or pWBT-pleD-cuxR (+) and were grown in liquid MM supplemented with 0.5 mM IPTG.
c
Values are from two independent biological replicates.
d
Sample dialysis with a 7,000-Da molecular weight cutoff membrane after ethanol precipitation.