TABLE 2.

Strains and plasmids used in this work

Strain or plasmid	Genotype or description	Reference or source
Strains
L. lactis IL1403	Plasmid-free strain, Trp+	51
L. monocytogenes EGD-e	Wild type, serovar 1/2a strain	Laboratory collection
E. coli DH5α	fhuA2 Δ(argF-lacZ)U169 phoA glnV44 ϕ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17	52
E. coli BL21	B F− ompT gal dcm lon hsdSB(rB− mB−) [malB+]K-12(λS)	53
E. coli LB650	F− thi lacZ gal rha ΔkdpFABC5 trkD1 ΔtrkH ΔtrkG Km50 Cm30	35
E. coli LB2003	ΔkdpABC5 kup1 (trkD1) ΔtrkA	37
Plasmids
pBAD33	Para Cm30	45
pBP370	pBAD33 cdaAlmo Cm30	This work
pBP371	pWH844 ktrC-ktrD (L. monocytogenes) Amp100a	This work
pBP372	pWH844 ktrAB (B. subtilis) Cm30	6
pBP373	pBAD33 cdaAlmo D171N Cm30	This work
pWH844	Plac His6× Amp100	48
pIQ309	pWH844 kupA Amp100	This work
pIQ310	pWH844 kupB Amp100	This work