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. Author manuscript; available in PMC: 2019 Apr 25.
Published in final edited form as: Nat Struct Mol Biol. 2018 Aug 27;25(9):841–849. doi: 10.1038/s41594-018-0114-9

Figure 5. ZZ and BD of p300 differentially modulate p300 activity.

Figure 5.

(a) Binding affinities of p300-BRPHZ for H31–31 peptide measured by tryptophan fluorescence (duplicate measurements). (b) Binding curves used to determine the Kd values by fluorescence. (c) Western blot analysis of histone acetylation levels in whole cell extract of H1299 cells stably expressing wild type FLAG-p300BRPHZT or the indicated mutants. (d) Quantification of the indicated histone acetylation levels from three biological replicates as in (c). Total H3 or H4 was used for normalization. (e) Western blot analysis of histone acetylation levels in whole cell extract of H1299 cells stably expressing the indicated FLAG-p300BRPHZT fragments. (f) Quantification of the indicated histone acetylation levels from three biological replicates as in (e). Total H3 or H4 was used for normalization. Error bars in (d, f) represent s.e.m. from 3 independent batches of stable cells (n = 3 biological replicates). Uncropped blot images (c, e) are shown in Supplementary Data Set 1.