Antibodies |
PSD-95 |
Chemicon |
MAB1596; RRID: AB_2092365 |
GABAAR 2/3 |
Millipore |
05–474; RRID: AB_309747 |
Gephyrin |
Abcam |
ab25784; RRID: AB_1209349 |
CaMKIIα |
Made in House |
CBα2 |
GluN2B |
PhosphoSolutions |
AB_2492176; RRID: AB_2492176 |
GluN2B pS1303 |
Millipore |
07–398; RRID: AB_310582 |
GluN1 |
BD Biosciences |
556308; RRID: AB_396353 |
Beta (III) Tubulin |
Millipore |
05–559; RRID: AB_309804 |
Rabbit |
GE Healthcare |
NA934V; RRID: AB_772206 |
Mouse |
GE Healthcare |
NA931V; RRID: AB_772210 |
Alexa-647 Anti-Rabbit |
Molecular Probes |
A21245; RRID: AB_2535813 |
Alexa-647 Anti-Mouse |
Molecular Probes |
A21236; RRID: AB_141725 |
Chemicals, Peptides, and Recombinant Proteins |
HiLyte Fluor 647-labeled Aβ1–42 peptide |
AnaSpec |
AS-64161 |
Unlabeled Aβ1–42 peptide |
AnaSpec |
AS-20276 |
Scrambled Aβ1–42 peptide |
AnaSpec |
AS-25382 |
Papain |
Worthington |
LS 03126 |
Lipofectamine 2000 |
Invitrogen |
11668027 |
B-27 supplement |
GIBCO |
17504044 |
KN-93 inhibitor |
Tocris |
139298–40-1 |
tatCN21 inhibitor |
Millipore |
5.32385.0001 |
cOmplete protease inhibitor cocktail (EDTA-free) |
Roche |
1187380001 |
Critical Commercial Assays |
Pierce BCA protein assay |
Thermo-Fisher |
23225 |
Recombinant DNA |
CaMKIIα-FingR-GFP |
Dr. Donald Arnold (USC) Mora et al., 2013
|
N/A |
PSD-95-FingR-GFP |
Addgene |
46295 |
Gephyrin-FingR-GFP |
Addgene |
46296 |
Software and Algorithms |
Slidebook 6.0 |
Intelligent Imaging Innovations (3i) |
N/A |
Prism 7.0 |
Graphpad |
RRID: SCR_002798 |
AlphaEase FC 4.0 |
Alpha Innotech |
N/A |