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. 2019 Mar 21;4(6):e123919. doi: 10.1172/jci.insight.123919

Figure 6. Treg expression of IL-13 reduces inflammatory cytokines and myeloid cell infiltration to protect against mortality after ALI.

Figure 6

(A) Survival of B6 Il33–/– mice instilled i.t. with bleomycin (1.0 IU/kg) and also treated with i.t. PBS (n = 10) or 1 μg recombinant (r) mouse IL-13 (n = 8). (B) Survival of Treg-depleted mice i.t. instilled with bleomycin (1.0 IU/kg) and 1 μg rIL-33 with or without rIL-13 (1 μg). (C) Weight changes of BALB/c Foxp3Cre mice (n = 7) injected i.t. with bleomycin (6.0 IU/kg) compared with bleomycin-treated Foxp3Cre × Il4/Il13fl/fl (n = 8) or PBS-treated Foxp3Cre mice (n = 3). Data were pooled from 2 separate experiments. (D) Survival of Foxp3Cre (n = 12) and Foxp3Cre × Il4/Il13fl/fl (n = 11) injected i.t. with bleomycin. (E) At day 7 after bleomycin delivery, BALF from Foxp3Cre × Il4/Il13fl/fl had increased IL-6, G-CSF, and MCP-1 concentrations. (F) Flow cytometric analysis of BALF cells at day 7 after bleomycin delivery for alveolar macrophages (CD45+CD11bloSiglec-F+), neutrophils (CD45+CD11bhiSiglec-F–Ly6Ghi), and inflammatory monocytes (CD45+CD11bhiCD24loMHC-IIloLy6Chi). In E and F, data were pooled from 2 independent experiments (n = 5–6 mice per group total). (G) qRT-PCR data of Arg1 expression in cultures of F4/80-purified BALB/c splenic macrophages (Il1rl1+/+ or Il1rl1–/–) incubated for 18 hours under the conditions indicated. Data are from 1 experiment representative of 3 completed. Animal survival was compared by Kaplan-Meier analysis and the log-rank test. Data are the mean ± SD and P values were determined by 2-tailed Student’s t test or 1-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.