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. 2019 Mar 21;4(6):e122686. doi: 10.1172/jci.insight.122686

Figure 5. Generation of induced pluripotent stem cells and cardiomyocytes from myotonic dystrophy subjects.

Figure 5

The same urine cells isolated from healthy control, DM1, and DM2 subjects were reprogrammed to induced pluripotent stem cells (iPSCs) using episomal delivery of Yamanaka factors. iPSCs were then differentiated into cardiomyocytes (iPSC-CM). To monitor differentiation status, cells were immunostained with antibodies to actin (green) and cardiac myosin binding protein C (cMyBP-C) (red). Nuclei were labeled with Hoechst (blue). DM1, DM2, and control cells successfully differentiated into iPSC-CMs and the cardiac markers, actin and MYBPC3, demonstrated the expected sarcomeric pattern. Higher-magnification images of the white dotted boxes are shown on the right column. Scale bar: 25 μm (all panels except region of interest). Scale bar: 10 μm (region of interest).