Fig. 3.

Isoproterenol activation of transmembrane adenylyl cyclase (AC) in pulmonary microvascular endothelial cells (PMVECs) increases cellular and extracellular vesicle (EV)-cAMP. PMVECs were stimulated for 15 min with or without isoproterenol in the presence or absence of rolipram and cell lysates (A and C) and EVs (B and D) analyzed for cAMP by either EIA (A and B) or mass spectrometry (C and D). cAMP was normalized to protein content (n = 3; ***P < 0.001, **P < 0.01, 1-way ANOVA with the Tukey post hoc test). ● and ○, Individual data points.