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. 2019 Feb 13;316(4):L691–L700. doi: 10.1152/ajplung.00282.2018

Fig. 6.

Fig. 6.

Transmission electron microscopy (TEM) analysis of extracellular vesicles (EVs). EVs isolated from either unstimulated or isoproterenol- (1 µM) and rolipram-stimulated (10 µM) (60 min) pulmonary microvascular endothelial cells were fixed and counterstained for TEM. EVs from both unstimulated (A and B) and isoproterenol- and rolipram-stimulated (C and D) pulmonary microvascular endothelial cells exhibit characteristics of intact, heterogenous vesicles. The EVs range in size from exosomes (≤150 nm) to microparticles (≥200 nm up to 1 μM). Representative images from 6 independent preparations for each condition.