Fig. 9.

Extracellular vesicles (EVs) from unstimulated (vehicle control) as well as isoproterenol and rolipram stimulated pulmonary microvascular endothelial cells (PMVECs) do not alter transendothelial electrical resistance. EVs collected (as described in materials and methods) from PMVECs stimulated with isoproterenol (1 µM) and rolipram (10 µM) or vehicle control for 60 min were applied to confluent PMVEC monolayers and the change in resistance recorded over time. DMEM was used as the EV vehicle control. EVs collected from vehicle control or isoproterenol- and rolipram-treated PMVECs did not alter monolayer resistance from vehicle control-treated PMVECs (data represent n = 4, with each individual experiment performed as n = 2 or 3; *P < 0.05, 2-way ANOVA with Tukey post hoc test).