Fig. 1.

Administration of U0126, a selective inhibitor of extracellular signal-regulated kinases 1 and 2, attenuates renal epithelial-mesenchymal transition (EMT) in kidney of hyperuricemic rats. HN, hyperuricemic nephropathy. A: kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against vimentin, E-cadherin, Snail1, and GAPDH. B–D: expression levels of vimentin (B), E-cadherin (C), and Snail1 (D) were quantified by densitometry, and protein levels were normalized to GAPDH. E: photomicrographs (×200) illustrating vimentin immunochemistry staining of kidney tissue. F: vimentin staining graphic presentation of quantitative data. Data are presented as mean ± SE (n = 6). Means with different superscript letters are significantly different from one another (P < 0.05). All scale bars, 20 μm.