Fig. 3.

Levels of p21 and p53 mRNA 4 and 18 h after glycerol (Glyc) injection and phosphorylated (Ser¹⁵) p53 (p53-ser15p) levels 4 h after glycerol injection. Marked elevations in p21 mRNA were observed 4 h after glycerol injection, despite a significant decrease in p53 mRNA levels at 4 h. C,  controls. By 18 h after glycerol injection, p21 mRNA increased further (note change in y-axis for 4 h vs. 18 h). Despite a small increase in p53 mRNA at 18 h, there was no correlation between p21 mRNA values and the corresponding p53 mRNA values (r = −0.02). Phosphorylated (Ser¹⁵) p53 protein increased 3- to 4-fold 4 h after glycerol injection. Values are means ± SE; n = 5–10 mice per group.