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. 2019 Jan 16;316(4):F732–F742. doi: 10.1152/ajprenal.00262.2018

Fig. 3.

Fig. 3.

Inhibition of 5-lipoxygenase (5-LO) reduces renal fibrosis after unilateral ureteral obstruction (UUO). A: microscopy for second-harmonic generation (SHG, red) and autofluorescence (green) in control kidneys and kidneys 7 days after UUO. B: quantification of SHG signal at 7 days (%fibrosis area) reveals a decrease in collagen in zileuton-treated mice (n = 5 mice/group). C and D: SHG/autofluorescence images from 14 days after UUO (n = 3 mice/group). E and F: hydroxyproline assays from homogenized kidneys at 7 and 14 days after UUO in zileuton-treated mice (n = 7 mice/group). G: hydroxyproline assay 14 days after UUO in 5-LO-associated protein knockout (Alox5ap−/−) and wild-type (WT) mice (n = 7 mice/group). Values are means ± SE. *P < 0.05; ns, not significant. Scale bars = 50 μm.