FIG 5.
Gal-3 disrupts P. brasiliensis extracellular vesicles. (A) Purified radiolabeled vesicles 72 h after [1-14C]palmitic acid addition were resuspended in PBS, BSA, denatured Gal-3, Gal-3 preincubated with lactosamine, or Gal-3 (0.001 to 10 μg/ml), and the release of radioactivity from vesicle pellets was assayed. Supernatant and pellet radioactivity was assessed and normalized to 100% radioactivity for each individual sample. (B) Hemocyanin, ovalbumin, concanavalin A (ConA), phytohemagglutinin E (PHA-E), phytohemagglutinin L (PHA-L), and bovine serum albumin (BSA) were used as controls. Bars represent the means ± SDs from triplicate samples from three representative experiment. **, P < 0.005, two-tailed Student’s t test.