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. Author manuscript; available in PMC: 2019 Apr 25.
Published in final edited form as: Methods Mol Biol. 2019;1884:57–72. doi: 10.1007/978-1-4939-8885-3_4

Fig. 2.

Fig. 2

Flow cytometric gating for analysis of human cells from EBV -infected mice. (a) Examples of light scatter gating. Use height versus area for the same light scatter parameter (e.g., side scatter, or SSC) to identify singlet events; use the area of two different light scatter parameters (e.g., forward scatter versus side scatter) to exclude dead cells and debris from the analysis. (b) Identification of human cells using antibodies against HLA and human CD45. (c) Identification of human T cells and B cells using antibodies against CD3 and CD19 and/or CD20