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. 2019 Apr 25;14(4):e0207968. doi: 10.1371/journal.pone.0207968

Fig 1. Phylogenetic analysis of homologous YxaL protein sequences.

Fig 1

A minimum evolutionary tree was constructed using the Close-Neighbor-Interchange algorithm at a search level of 1, based on the Dayhoff model with 1000 bootstrap replications in MEGA 7. The Neighbor-Joining algorithm was used to generate the initial tree. The analysis involved 100 amino acid sequences. All ambiguous positions (gaps) generated from a multiple sequence alignment using Clustal Omega were removed for each sequence pair. There were a total of 630 positions in the final dataset and the evolutionary tree was drawn with branch lengths, as shown in S1 Fig. The position of YxaL obtained from Bacillus velezensis strain GH1-13 is marked by a circle symbol before prefixing the strain name with the abbreviated taxonomic name. Abbreviations used: Bamy, B. amyloliquefaciens; Bhal, B. halotolerans; Bnak, B. nakamurai; Bsiam, B. siamensis; Bsub, B. subtilis; Bteq, B. tequilensis; Bvel, B. velezensis; Ecoli, Escherichia coli; Mabs, Mycobacteroides abscessus subsp. massiliense; and Mtub, Mycobacterium tuberculosis.