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. 2019 Apr 25;14(4):e0209813. doi: 10.1371/journal.pone.0209813

Table 2. Sediment filters tested, numbers and dimensions of nematodes pre- and post-filtration, qPCR results, including nematode loss controls for the test processes and vacuum filtration.

Brand Filter # # of nematodes post/pre (% of nematodes that traversed the filter) Real-time PCR (# nematodes tested) Nematode length/ diameter (μm) % of nematodes with L3 length pre/post
Run 1 Run 2 Run 3 Run 4 Total Pre-filter Post-filter
20 μm wound polypropylene, United Filters International (UP20R10P) 1 10/250 (4%) 17/250 (6.8%) 37/500 (7.4%) 0/0 64/1000 (6.4%) + (50) 188-606/ 10–34 196-741/ 8–29 73% / 63%
2 12/250 (4.8%) 18/250 (7.2%) 31/500 (6.2%) 6/0 67/1000 (6.7%) + (67) 342-520/ 17–27 390-495/ 20–26 96% / 58%
10 μm wound polypropylene, Culligan (CW-F) 1 37/250 (14.8%) 26/250 (18.4%) 99/500 (19.8%) 23/0 185/1000 (18.5%) + (82) 227-540/ 11–35 394-529/18-28 91% / 84%
2 108/250 (43.2%) 98/250 (39.2%) 185/500 (37%) 22/0 413/1000 (41.2%) + (413) 383-516/ 18–29 250-528/ 13–28 99% / 81%
5 μm carbon block, Matrikx Accucarb (32-250-10-GREEN) 1 0/250 (0%) 0/250 (0%) 0/500 (0%) 0/0 0/1000 (0%) n/a 399-530/ 18–33 n/a 96% / n/a
2 0/250 (0%) 0/250 (0%) 0/500 (0%) 0/0 0/1000 (0%) n/a 360-540/ 17–42 n/a 96% / n/a
5 μm spun polypropylene, Culligan (P5) 1 0/250 (0%) 0/250 (0%) 6/500 (1.2%) 9/0 15/1000 (1.5%) + (15) 185-1374/ 10–68 192-606/ 9–36 73% / 53%
2 0/250 (0%) 0/250 (0%) 1/500 (0.2%) 3/0 4/1000 (0.4%) + (4) 239-1440/ 13–78 374-755/ 16–34 46% / 25%
3 0/250 (0%) 0/250 (0%) 0/500 (0%) 1/0 1/1000 (0.1%) - (1) 342-887/ 12–34 398/ 16 96% / n/a
1 μm spun polypropylene, Culligan (P1) 1 0/250 (0%) 0/250 (0%) 0/500 (0%) 64/0 64/1000 (6.4%) + (64) 372-512/ 19–28 n/a 94% / n/a
2 0/250 (0%) 0/250 (0%) 0/500 (0%) 0/0 0/1000 (0%) n/a 327-553/ 18–28 n/a 93% / n/a
3 0/250 (0%) 0/250 (0%) 0/500 (0%) 9/0 9/1000 (0.9%) + (9) 393-510/ 19–27 432-492/ 20–24 97% / 100%
Nematodes control testing   200/250 (80%) 168/250 (67%) 160/250 (64%)            
Nematode control vacuum filtration   80/100 (80%) 86/100 (86%) 71/100 (71%) 77/100 (77%)