Table 1. RT-LAMP and RT-PCR primer sequences.
Tests | Target gene | Primer name | Sequence (5’ to 3’) |
---|---|---|---|
RT-LAMP | Pfs16 | F3 | CTTCGCTTTTGCAAACCTG |
B3 | CTAGCTGAGTTTCTAAAGGCAT | ||
FIP (F1c-F2) | GGTTTGCAAAGTTGAAGGGGATCAGATGCAAATGACAAAGCA | ||
BIP (B1c-B2) | CAGGAAGTTCTTCAGGTGCCTCACCTTGAGATAGTCCACCTT | ||
Loop F | CTTTTCCAGCGGGCTTTT | ||
Loop B | TCTTCATGCTGTTGGACCTAAT | ||
RT-PCR | Pfs16 | F3 | CTTCGCTTTTGCAAACCTG |
B3 | CATCTCCTTCGTCTCCTTCATC | ||
Pfs25 | F3 | AAGTTACCGTGGATACTGTATG | |
B3 | TGAGCATTTGGTTTCTCCAT |
Pfs16: Plasmodium falciparum sexual stage 16 kD; Pfs25: Plasmodium falciparum sexual stage 25 kD