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. 2019 Apr 25;14(4):e0216133. doi: 10.1371/journal.pone.0216133

Table 1. RT-LAMP and RT-PCR primer sequences.

Tests Target gene Primer name Sequence (5’ to 3’)
RT-LAMP Pfs16 F3 CTTCGCTTTTGCAAACCTG
B3 CTAGCTGAGTTTCTAAAGGCAT
FIP (F1c-F2) GGTTTGCAAAGTTGAAGGGGATCAGATGCAAATGACAAAGCA
BIP (B1c-B2) CAGGAAGTTCTTCAGGTGCCTCACCTTGAGATAGTCCACCTT
Loop F CTTTTCCAGCGGGCTTTT
Loop B TCTTCATGCTGTTGGACCTAAT
RT-PCR Pfs16 F3 CTTCGCTTTTGCAAACCTG
B3 CATCTCCTTCGTCTCCTTCATC
Pfs25 F3 AAGTTACCGTGGATACTGTATG
B3 TGAGCATTTGGTTTCTCCAT

Pfs16: Plasmodium falciparum sexual stage 16 kD; Pfs25: Plasmodium falciparum sexual stage 25 kD