Fig 5. The signatures of BIM cells and BD-ILC1 cells are reproducible at the tissue level and restricted to the extrahepatic bile duct.

RNA sequencing was performed on liver or bile duct tissue isolated from mice treated with either PBS or IL-33 for 4 days. BIM-specific genes (A) and BD-ILC1 specific genes (B) are selectively upregulated in the EHBD after IL-33 treatment (4d BD) relative to IL-33 treated liver (4d Liver) and PBS control tissues. N = 3 samples per group. Data are presented as mean ± s.d., * = p<0.05, ** = p<0.005 by one-tailed Student’s t-test. The gene expression signatures of the remaining 4 major cell classes were not reproduced at the tissue level.