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. 2019 Mar 7;4(5):e122943. doi: 10.1172/jci.insight.122943

Figure 6. Platelet β2M regulates circulating monocyte differentiation.

Figure 6

(A) Circulating monocytes were isolated from WT and Plt-β2M–/– mice, and proinflammatory and proreparative monocyte gene markers were quantified by qPCR. Plt-β2M–/– mouse monocytes had proreparative monocyte gene expression (all panels, n = 3; *P < 0.05, unpaired 2-tailed t test with Welch’s correction). (B and C) Monocytes from Plt-β2M–/– mice differentiate to proreparative, fibroblast activating, macrophages in vitro. Peripheral blood monocytes from WT and Plt-β2M–/– mice were coincubated with cardiac fibroblasts for 72 hours and macrophage differentiation (B) and fibroblast activation (C) were determined by qPCR, and IL-10 secretion determined by ELISA. Plt-β2M–/––derived monocytes had increased proreparative differentiation and IL-10 secretion and induced more fibroblast activation compared with WT mouse monocytes (B and C, n = 4; *P < 0.05, **P < 0.01 vs. WT, unpaired 2-tailed t test with Welch’s correction).