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. 2019 Mar 7;4(5):e126742. doi: 10.1172/jci.insight.126742

Figure 2. Proglucagon products are necessary for nutrient-stimulated insulin secretion.

Figure 2

(A) Insulin secretion in response 10 mM glucose, 10 mM glutamine, 1 mM arginine, 10 nM glucagon, 3 nM GIP, and 0.3 nM GLP-1 from WT or Gcgnull islets (n = 7). (B) Insulin secretion in response 10 mM glucose, 10 mM glutamine, 1 mM arginine, 10 nM glucagon, 3 nM GIP, and 0.3 nM GLP-1 from WT + Ad-CMV-Cre (n = 3) or Gcgnull + Ad-CMV-Cre islets (n = 5). (C) Insulin secretion in response to 10 mM glucose, 10 nM glucagon, and 3 nM GIP from WT islets with or without 1 μM Ex9 and 10 μg/ml GRA (n = 6). (D) Insulin secretion in response to 10 mM glucose, 10 mM glutamine, and 1 mM arginine from WT islets with or without 1 μM Ex9 and 10 μg/ml GRA (n = 6). *P < 0.05. Data are shown as mean ± SEM. Data were analyzed by a 2-way ANOVA of the iAUCs.