Table 1. The kinetic parameters and affinity constants of mAbs 3G11 and 5H5 for binding with linear tri- (G3), penta- (G5) and nonaglucoside (G9) and branched octaglucoside brG8.
mAb | Synthetic glucosides | |||
---|---|---|---|---|
G3 | G5 | brG8 | G9 | |
3G11 | KD > 1 mM | KD = 12 ± 1 nM | KD > 1 mM | KD = 11 ± 1 nM |
ka = 6.4 × 104 M-1s-1 | ka = 8.2 × 104 M-1s-1 | |||
kd = 8.5 × 10−4 s-1 | kd = 8.8 × 10−4 s-1 | |||
5H5 | KD = 9.0 ± 3.0 nM | KD = 4.0 ± 0.5 nM | KD = 4.5 ± 0.5 nM | KD = 1.9 ± 0.2 nM |
ka = 7.2 × 104 M-1s-1 | ka = 1.4 × 105 M-1s-1 | ka = 1.7 × 105 M-1s-1 | ka = 4.2 × 105 M-1s-1 | |
kd = 6.3 × 10−4 s-1 | kd = 5.6 × 10−4 s-1 | kd = 7.8 × 10−4 s-1 | kd = 8.1 × 10−4 s-1 |