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. 2019 Mar 7;4(5):e125914. doi: 10.1172/jci.insight.125914

Figure 2. aMVECs take up and release lipids but are not adipose precursor cells.

Figure 2

(A–E) Lipolytic effect of forskolin in aMVECs: Cells were treated for 24 hours without (BAS) or with 300 μM OA. During the last 24 hours of incubation, OA was removed (B and D) or not (E), and the cells were exposed to 10 μM forskolin for an additional 24 hours (C and D). The intracellular lipid accumulation was revealed by Oil Red O staining (original magnification, ×100). (F–N) aMVECs are not adipose precursor cells. PAs and aMVECs were incubated with or without an adipogenic differentiation (Diff) cocktail for 7 days. (F–I) The presence of lipids was revealed with Oil Red O staining (original magnification, ×100). (J–N) The genes, as indicated in the figures, were analyzed with qRT-PCR. Data are from at least 4 experiments. **P < 0.01. Bars represent mean ± SEM. Wilcoxon’s signed-rank test.