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. 2019 Apr 4;4(7):e125019. doi: 10.1172/jci.insight.125019

Figure 3. miRs carried by EVs are differentially present in OA synovial fluid from young versus aged mice after clearance of SnCs.

Figure 3

(A) Quantification of miR-34a-5p, miR-128a-3p, and miR-146a-5p in young and aged PTOA mice treated with vehicle (veh) or the senolytic UBX0101, which can mediate senescence and the SASP, 28 days after ACLT surgery. All data are expressed as mean ± SEM, and each data point represents an individual mouse. One-way ANOVA with Tukey’s multiple-comparisons test was used for statistical analysis (young, n = 4; aged, n = 5). *P < 0.05. (B) Workflow of analysis of EVs from the synovial fluid of PTOA mice treated with veh or UBX0101. (C and D) Plots illustrating the fold change (UBX0101/veh; x axis) and significance level expressed as the log P value (y axis). The blue circles represent miRs that were upregulated and red circles represent miRs that were downregulated by UBX0101 compared with veh-treated PTOA young (C) and aged (D) mice (n = 3 per group). Significance was determined based on a P value cutoff of 0.05. (E) The heatmap reveals significant correlations among mmu-miR-30c-5p, -92a-3p, -24-3p, -186-5p, -125a-5p, and -150-5p, expression of which was significantly altered by UBX0101 treatment in young PTOA mice and the signaling pathways in which they are predicted by the DIANA-miRPath (v3.0) to participate.