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. 2019 Apr 4;4(7):e125489. doi: 10.1172/jci.insight.125489

Figure 4. mATG lymphoablation induces B cell cytokine production in a CD4-dependent manner.

Figure 4

(A) Revised model. (B and C) C57BL/6J mice were transplanted with BALB/c heart allografts and either left untreated (N.T.), treated with mATG alone (mATG), or with mATG and depleting anti-CD4 mAb (mATG + αCD4). RNA was isolated from flow-sorted splenic B cells and gene expression was analyzed by NanoString nCounter Gene Expression Assay (B) and real-time RT-PCR (qPCR) (C). Data represent fold increase in expression relative to B cells isolated from naive C57BL/6J mice. The results are representative of 2 experiments with n = 4 mice/group; error bars represent SD. *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA with Tukey’s multiple-comparisons post hoc test.