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. 2019 Apr 4;4(7):e124698. doi: 10.1172/jci.insight.124698

Figure 5. The PCPC population differentiates into PCs.

Figure 5

(A–C) Naive B cells (B220+CD19+IgM+CD138–AA4.1–), PCPCs, and PCs (B220–CD138+) were sorted from XBP1-Tg mice and adoptively transferred into μMT mice (n = 4 mice/group). (A) FACS staining profile of nucleated cells before sorting. (B) Characterization of naive, PCPC, and PC populations after sorting but before the adoptive transfer. The percentages of each population in the final sorted cellular products are indicated. (C) Total IgG1 and IgM concentrations in the sera of recipient mice were measured by ELISA 4 months after the adoptive transfer. *P < 0.05 and error bars denote ± SEM. (D) PCs (B220–CD138+), BCPCs, PCPCs, and memory-like B cells (PCPC sIgG+) were sorted to over 90% purity and placed into PC-culturing conditions for 7 days. The total numbers of PCs generated from 300,000 total cells are shown (n = 4). In C and D, 1-way ANOVA with Tukey’s multiple-comparisons test was used to calculate *P < 0.05. All error bars denote ± SEM.