Figure 3. CXCL9 and CXCL10 are produced by resident and recruited cardiac myeloid cells in response to cardiac pressure overload.

REX3 mice were subjected to TAC surgery, and LV tissue sections were isolated for analysis of CXCL9 and CXCL10 expression by flow cytometry, 4 weeks after TAC. (A and B) CD11b⁺Ly6G⁺ Neutrophils, CD11b⁺MerTK^–CCR2⁺ monocytes, CD11b⁺MerTK⁺CCR2^– resident macrophages, and CD11b⁺MerTK⁺CCR2⁺ recruited macrophages were identified by flow cytometry with the indicated gating strategy. (C–F) CXCL9 and CXCL10 expression was quantified as frequency (C and D) and absolute cell number per LV (E and F). Statistical comparisons are indicated compared with neutrophils. n = 3 mice per group. Error bars represent mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001; 1-way ANOVA with Bonferroni post hoc test).