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. 2019 Apr 4;4(7):e125107. doi: 10.1172/jci.insight.125107

Figure 5. cGAMP enhances the induction of protective antitumor CD8+ T cells in vivo.

Figure 5

C57BL/6J mice were vaccinated intramuscularly with PBS (negative control) or OVA, either alone or together with various adjuvants, on days 0 and 14. (A) Top: Representative flow cytometry data showing SL8-specific CD8+ T cells 1 week after the last vaccination. Plots are gated on viable CD3+ events. Percentage values refer to H-2Kb-SL8 tetramer+ cells among total CD8+ T cells. Bottom: Data summary (n = 4 mice per group). (B) Left: Representative flow cytometry data showing IFN-γ production among CD8+ T cells restimulated with SL8 1 week after the last vaccination. Plots are gated on viable CD3+ events. Percentage values refer to IFN-γ+ cells among total CD8+ T cells. Right: Data summary. (C) Tumor size after subcutaneous injection of EG7 cells on day 21 (n = 10 mice per group). Error bars indicate mean ± SEM. *P < 0.05, **P < 0.01 (unpaired t test).