Skip to main content
. 2019 Apr 4;4(7):e125107. doi: 10.1172/jci.insight.125107

Figure 6. cGAMP enhances the induction of protective antiviral CD8+ T cells in vivo.

Figure 6

CB6F1 mice were vaccinated intranasally with PBS (negative control) or NP-p24, either alone or together with 2′3′-cGAMP, on days 0, 7, 14, and 21. (A) Frequency of H-2Kd-AI9 tetramer+ CD8+ T cells 1 week after the last vaccination. (B) Frequency of IFN-γ–producing cells among splenocytes restimulated with AI9 1 week after the last vaccination. Results are expressed as the number of spot-forming units (SFU) per 3 × 105 splenocytes. (C) Weight loss after challenge with VV-Gag. Unchallenged naive mice were included as a negative control. (D) Viral titers 1 week after challenge with VV-Gag. Error bars indicate mean ± SEM ( n = 5 mice per group). *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired t test or Mann-Whitney U test).