Figure 2. Effect of S1P inhibition alone or in combination with sPRR-His on AVP-induced V₂R and AQP2 expression in the CD cells.

Primary rat IMCD cells were pretreated with PF alone or in combination with sPRR-His and then treated for 24 hours with 10 nM AVP. The expression of V₂R and AQP2 were analyzed by immunoblotting. Medium sPRR concentration was determined by using ELISA and normalized by protein content. (A) Immunoblotting analysis of V₂R and AQP2 expression (n = 3 per group). Densitometry values are shown underneath the blots. (B) ELISA analysis of medium sPRR content (n = 6 per group). Statistical significance was determined by using 1-way ANOVA with the Bonferroni test for multiple comparisons. *P < 0.05 versus control (CTR); ^#P < 0.05 vs. PF-429242 alone, ^&P < 0.05 vs. AVP.