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. 2019 Apr 4;4(7):e124174. doi: 10.1172/jci.insight.124174

Figure 3. In vivo characterization of the diuretic action of S1P inhibition in C57/BL6 mice.

Figure 3

Male C57/BL6 mice received s.c. infusion of a S1P inhibitor, PF-429242 (PF), with or without sPRR-His infusion for 4 days. The mice receiving vehicle treatment served as control. At the end of the experiment, 24-hour urine collection was performed, followed by ELISA analysis of urinary sPRR excretion. (A) ELISA analysis of urinary sPRR excretion (n = 10 mice per group). (B) Urine volume (n = 10 mice per group). (C) Urinary osmolality (n = 10 mice per group). (D) Analysis of urine osmolality response to acute AVP treatment (n = 10 mice per group). (E) ELISA analysis of plasma AVP concentration (n = 6 mice per group). (F) Plasma osmolality (n = 6 mice per group). Statistical significance was determined by using 1-way ANOVA with the Bonferroni test for multiple comparisons. *P < 0.05 vs. control; #P < 0.05 vs. PF alone.