Figure 4. Effect of SIP inhibition alone or in combination with sPRR-His on renal expression of V₂R, transporter proteins, and autophagosome markers in C57/BL6 mice.

Male C57/BL6 mice were treated with vehicle, PF alone, or in combination with sPRR-His. The renal expression of V₂R, AQP2, NKCC2, and the markers of autophagosome (p62 and LC3b) was analyzed by immunoblotting. (A) Immunoblotting analysis of V₂R and AQP2 expression (n = 8 mice per group). (B) Immunoblotting analysis of NKCC2, p62, and LC3b expression (n = 8 mice per group). Densitometry values are shown underneath the blots. Statistical significance was determined by using unpaired Student’s t test. *P < 0.05 versus control (CTR); ^#P < 0.05 vs. PF-429242 alone.