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. 2019 Apr 4;4(7):e124174. doi: 10.1172/jci.insight.124174

Figure 6. Analysis of fluid homeostasis in RT S1P–KO mice and rescue of the phenotype with sPRR-His.

Figure 6

At the age of 3–4 months, RT S1P–KO mice were infused with vehicle or sPRR-His infusion via osmotic minipump at 30 μg/kg/day for 7 days. At the end of the experiments, 24-hour urine collections were performed and urine osmolality response to acute AVP injection was determined. (A) Urine volume (n = 8 mice per group). (B) Urine osmolality (n = 8 mice per group). (C) Ratio of the change in urine osmolality response to acute AVP treatment. Urine was emptied by bladder massage and subjected to measurement of osmolality before and after AVP (720 ng/kg body weight) treatment (n = 8–9 mice per group). Statistical significance was determined by using 1-way ANOVA with the Bonferroni test for multiple comparisons. Data are mean ± SEM. *P < 0.05 vs. control; #P < 0.05 vs. RT S1P–KO mice alone.