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. 2019 Apr 4;4(7):e125754. doi: 10.1172/jci.insight.125754

Figure 4. Exosomal miR-148a promotes mdx skeletal muscle regeneration.

Figure 4

(A) Amplification plot from a qPCR analysis of miR-148a in human cardiosphere-derived cells (CDCs) and their exosomes (EXOs). EXOs contain an 8-fold higher abundance of miR-148a than their parent CDCs. (B) Schematic of the experimental design. (C) qPCR analysis of miR-148a expression in mdx solei 48 hours after injection of RNA scramble or anti–miR-148a. Anti–miR-148a–injected solei contained 5-fold less miR-148a than RNA scramble–injected solei. Fold change was calculated using the 2–ΔΔCt method. Error bar reflects SEM of pooled ΔCt data (n = 3). (D) Representative hematoxylin and eosin–stained micrographs from RNA scramble control– and anti–miR-148a–injected mdx solei after 3 weeks. Scale bars: 100 μm. (E) Pooled data from D reveal more total myofibers in the RNA scramble–injected solei than in the anti–miR-148a–injected solei (n = 5). (F) Immunohistochemical staining for neonatal myosin heavy chain (nMHC) and laminin in RNA scramble control–injected and anti–miR-148a–injected mdx solei after 3 weeks. Scale bars: 50 μm. (G) Pooled data from E reveal more nMHC+ myofibers in RNA scramble–injected solei than in the anti–miR-148a–injected solei (n = 5 per group). Bar graphs depict mean ± SEM. Statistical significance was determined by independent t tests with P ≤ 0.05. *Significantly different from RNA scramble control.