Herbrecht 2002.

Study characteristics
Patient sampling	Consecutive ("all patients presenting with ... had a serum sample collected") and prospective series of patients Episode‐based analysis
Patient characteristics and setting	177 participants Age ranged from 4 months to 88 years France Neutropenic children and adults (neutrophil count < 500/ųl) with a persistent fever despite antibiotics, but without any other signs of infection Daily monitoring of clinical course
Index tests	Platelia: sera with an index > 1.5 ODI were considered positive
Target condition and reference standard(s)	Invasive aspergillosis; EORTC‐criteria, citation Ascioglu 2002 The results of the antigen testing were not included in our classification
Flow and timing	All patients fulfilling the inclusion criteria were diagnosed with the EORTC criteria Withdrawals explained? No. Not clear how they came from 797 episodes to 640 episodes Uninterpretable results reported? Not reported
Comparative
No patients per category	6 proven, 3 probable, 75 possible, 93 non‐IA
Notes	Nothing reported on sponsoring
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Yes
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Yes
		Low	Low
DOMAIN 2: Index Test All tests
Were the index test results interpreted without knowledge of the results of the reference standard?	Unclear
If a threshold was used, was it pre‐specified?	Yes
		Unclear	Low
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Yes
		Low	Low
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Unclear
		Unclear