Machetti 1998.

Study characteristics
Patient sampling	22 allogeneic BMT patients were followed from transplant to 90 days onwards; 5 developed IA. No information on how the 22 were selected
Patient characteristics and setting	22 participants undergoing allogeneic bone marrow transplantation (BMT) No information about age or gender Setting: haematology department Country: Italy Inpatients; monitoring clinical course No further details
Index tests	Platelia Serum samples were collected 3 times a week during the first month and once a week during the second and third month Positivity was defined as at least 2 consecutive positive samples. One or less positive was considered negative Cut‐off: positive if > 1.5 ODI and negative if < 1.0 ODI
Target condition and reference standard(s)	Invasive aspergillosis, as defined by EORTC‐like criteria Galactomannan test was not mentioned as part of the reference criteria
Flow and timing	Until day 90 after transplantation All patients were classified according to the same reference criteria No withdrawals reported and no intermediate results reported
Comparative
No patients per category	1 proven, 3 probable, 1 possible, 17 no IA
Notes	Sponsoring precluded? No: ELISA was kindly provided by Sanofi Pasteur and they received financial support from Pfizer
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Unclear
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Unclear
		Unclear	Unclear
DOMAIN 2: Index Test All tests
Were the index test results interpreted without knowledge of the results of the reference standard?	Unclear
If a threshold was used, was it pre‐specified?	Yes
		Unclear	Low
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Unclear
		Unclear	Low
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
		Low