Moragues 2003.

Study characteristics
Patient sampling	Retrospective study, not much reported
Patient characteristics and setting	54 participants No information about age or gender Spain Severe neutropenic patients in the haematological department Monitoring clinical course, inpatients Representative spectrum? Unclear: severe neutropenic patients in the haematological department, without any further description
Index tests	Platelia. Sampled twice a week. 2 consecutive positive samples was considered as a positive result. No explanation of negative result. Cut‐off 1.5 ODI
Target condition and reference standard(s)	Invasive aspergillosis, as defined by EORTC criteria (Ascioglu 2002) Incorporation avoided? Yes: the results of the ELISA were not used for the reference criteria Acceptable reference standard? Yes: EORTC criteria (Ascioglu 2002)
Flow and timing	Time interval not reported Partial verification avoided? Yes: all patients were classified according to reference criteria Withdrawals explained? Yes Uninterpretable results reported? No
Comparative
No patients per category	3 proven, 1 probable, 17 possible, 33 no IA
Notes	Sponsoring? Financed by university Article in Spanish
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Unclear
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Unclear
		Unclear	Unclear
DOMAIN 2: Index Test All tests
Were the index test results interpreted without knowledge of the results of the reference standard?	Unclear
If a threshold was used, was it pre‐specified?	Yes
		Low	Low
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Yes
Were the reference standard results interpreted without knowledge of the results of the index tests?	Yes
		Low	Low
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Unclear
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Yes
		Low