Williamson 2000.

Study characteristics
Patient sampling	Prospective; consecutive series of patients; blood samples re‐analysed later for ELISA
Patient characteristics and setting	104 participants Age ranged from 3 months to 56 years No information about gender UK Children and adults undergoing bone marrow transplantation (BMT) or chemotherapy for haematological malignancy with severe neutropenia Inpatients Monitoring clinical course
Index tests	Platelia Aspergillus Serum samples were collected and tested twice weekly One positive is positive; no positive at all is negative Cut‐off value not reported
Target condition and reference standard(s)	Invasive aspergillosis, as defined by EORTC‐like criteria, no reference; 3 groups Galactomannan ELISA was not mentioned as part of the reference criteria
Flow and timing	All patients were classified according to the same reference criteria Withdrawals explained? Yes Uninterpretable results reported? No
Comparative
No patients per category	7 proven, no probables, 9 possible, 88 no IA
Notes	Nothing reported on conflicts of interest or sponsoring
Methodological quality
Item	Authors' judgement	Risk of bias	Applicability concerns
DOMAIN 1: Patient Selection
Was a consecutive or random sample of patients enrolled?	Yes
Was a case‐control design avoided?	Yes
Did the study avoid inappropriate exclusions?	Unclear
		Unclear	Low
DOMAIN 2: Index Test All tests
Were the index test results interpreted without knowledge of the results of the reference standard?	Yes
If a threshold was used, was it pre‐specified?	Unclear
		Unclear	Low
DOMAIN 3: Reference Standard
Is the reference standards likely to correctly classify the target condition?	Unclear
Were the reference standard results interpreted without knowledge of the results of the index tests?	Yes
		Unclear	Unclear
DOMAIN 4: Flow and Timing
Was there an appropriate interval between index test and reference standard?	Yes
Did all patients receive the same reference standard?	Yes
Were all patients included in the analysis?	Unclear
		Low