Figure 1. T cell adoptive transfer protocols for the assessment of autoimmune arthritis.

(A) Naïve wild type CD45.1⁺ GPI/I-A^g7–specific KRN CD4 T cells (10⁴) were adoptively transferred into CD45.2⁺ wild type and Tcra^–/– B6G7F1 (F1) hosts. After 24 hours, mice were injected i.p. twice daily with CGS 2.5 mg/kg or with the vehicle alone (PBS) until the endpoint at day 10. (B) Naïve CD45.1⁺ KRN CD4 T cells were isolated from both CD4-Cre Adora2a^f/f conditional knock-out (Adora2a^–/–) as well as wild type donor KRN mice, and then the T cells adoptively transferred (10⁴) into CD45.2⁺ Tcra^–/– F1 hosts to induce autoimmune arthritis. Mice were subsequently treated twice daily with either CGS or PBS alone for the duration of the experiment (10 d). (C) Naïve wild type CD45.1⁺ KRN CD4 T cells were adoptively transferred into CD45.2⁺ Tcra^–/– F1 hosts to induce arthritis. Beginning on day 8, mice were injected i.p. twice daily with CGS 2.5 mg/kg in PBS or else PBS alone as a control for the remainder of the experiment (with endpoints on days 8, 10, and 12 as indicated).