. Author manuscript; available in PMC: 2019 Nov 1.

Published in final edited form as: Nat Rev Mol Cell Biol. 2019 May;20(5):303–320. doi: 10.1038/s41580-019-0103-9

TABLE 1:

Transgenic mice for labelling of adult bone marrow HSPCs.

Mouse strain	Genetic modification	Specificity within the adult haematopoietic compartment	Analysis	Refs
Hoxb5-Tri-mCherry	Knock in	Specific to long-term HSCs	• Modified CUBIC clearing and light-sheet microscopy • Flow cytometry • Transplantation	²⁴³
Ctnnal1–GFP	Knock in	Restricted to HSCs and haematopoietic progenitors; requires KIT staining to enrich for HSCs	• Modified Murray’s clearing, immunostaining and confocal and multi-photon microscopy • Flow cytometry • Transplantation	⁴⁷
Fgd5–mCherry	Knock in	Restricted to HSCs; low expression in haematopoietic progenitors	• Flow cytometry • Transplantation	²⁴⁶
Vwf–GFP	Transgenic	Labels platelet/myeloid-biased HSCs, megakaryocyte progenitors, megakaryocytes and platelets	• Flow cytometry • Transplantation • Immunostaining and whole-mount confocal microscopy	^29,32
Msi2–GFP	Knock in	Also labels haematopoietic progenitors	• Confocal microscopy • Flow cytometry	²⁴⁷
Pdzk1ip1–GFP	Transgenic	Enriches for highly purified HSCs but also labels a small subpopulation of haematopoietic progenitors and mature granulocytes	• Doxycycline chase • Transplantation • Flow cytometry	¹⁶
Evi1–GFP	Knock in	Also labels haematopoietic progenitors	• Flow cytometry • Transplantation	²⁴⁸
Scl-tTA-H2B–GFP	Transgenic	H2B-GFP^high label-retaining cells are enriched in quiescent long-term HSCs; also labels haematopoietic progenitors	• Doxycycline chase • Transplantation • Flow cytometry • Immunostaining and confocal microscopy	^6,249
Tie2–GFP	Transgenic	Enriches for highly purified HSCs but also labels haematopoietic progenitors	• Flow cytometry • Transplantation • Immunostaining and whole-mount confocal and multi-photon microscopy	²⁴⁵
Gprc5c–GFP	Transgenic	Enriches for dormant HSCs but also labels haematopoietic progenitors	• Flow cytometry • Transplantation	²⁵⁰
Hdc–GFP	Transgenic	Enriches for myeloid-biased HSCs but also labels haematopoietic progenitors and mature myeloid cells	• Flow cytometry • Transplantation • Immunostaining and confocal microscopy	²⁵¹
Krt7–GFP	Knock in	Specific to HSCs	• Flow cytometry	²⁵²
Gata2–GFP	Knock in	Enriches for HSCs and haematopoietic progenitors; SCA1 or Lineage staining are required for HSC selectivity	• Flow cytometry • Transplantation • Immunostaining and microscopy	⁴⁴
Hoxb4–YFP	Knock in	Also labels haematopoietic progenitors	• Flow cytometry • Transplantation	²⁵³

Ctnnal1, α-catulin; CUBIC, clear, unobstructed brain imaging cocktails and computational analysis; Evi1, ecotropic virus integration site 1 homologue; Fgd5, FYVE, RHOGEF and PH domain-containing 5; Gata2, GATA-binding 2; Gprc5c, G-protein coupled receptor family C group 5 member C; H2B, histone H2B; Hdc, histidine decarboxylase; HSCs, haematopoietic stem cells; HSPCs, haematopoietic stem and progenitor cells; Hoxb5, homeobox b5; Krt7, keratin 7; Msi2, Musashi 2; Pdzk1ip1, PDZK1-interacting 1; Scl, stem cell leukaemia; Tie2, tyrosine kinase with immunoglobulin and EGF homology domains 2; tTA, tetracycline-controlled transactivator protein; Vwf, von Willebrand factor.