Fig. 7.

Amyloid protein precursor levels are correlated to Aβ peptides concentrations in microvessel extracts. Amyloid protein precursor (APP) levels in microvessel extracts were determined by Western blot. Data were normalized with cyclophilin B. a-c) Participants were divided according to a) their AD neuropathological diagnosis; b) their clinical diagnosis and c) their apoE4 allele carriage. No difference was observed in each of these comparisons. All samples, loaded in a random order, were run on the same immunoblot experiment. Consecutive bands were taken for the representative photo example. Data are represented as scatterplots. Horizontal bars indicate mean ± S.E.M. Statistical analysis: Mann Whitney test and Kruskal-Wallis ANOVA; p > 0.05. Linear regression analyses revealed that APP levels were positively correlated to both Aβ40 (panel e) and Aβ42 (panel f) concentrations in microvessel extracts. Statistical analysis: Pearson correlation coefficient. ¶ p < 0.05, ¶¶ p < 0.01. Abbreviations: −, ApoE4 non-carrier; +, ApoE4 carrier; A/AD, Alzheimer’s disease; ABC Dx, ABC neuropathological diagnosis; APP, amyloid protein precursor; C, control; Clinical Dx, clinical diagnosis; cypB, cyclophilin B; M/MCI, mild cognitive impairment; N/NCI, healthy controls with no cognitive impairment; relative O.D., relative optical density