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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Inherit Metab Dis. 2019 Feb 22;42(3):470–479. doi: 10.1002/jimd.12069

Fig. 2.

Fig. 2.

Characterization of pSVL-hG6Pase-α variants harboring the non-conserved amino acids in canine G6Pase-α. (a) The hG6Pase-α enzyme is anchored in the ER membrane by 9 helices (H1 to H9), creating 4 cytoplasmic (C1 to C4) and 4 luminal (L1 to L4) loops. The amino-terminus is located in the ER lumen and the carboxyl-terminus in the cellular cytoplasm. The non-conserved amino acids in hG6Pase-α differing from that in canine G6Pase-α are indicated and shown in black. The Cys residues are shown in grey and indicated by arrow heads. (b) Phosphohydrolase activity of WT and variant hG6Pase-α after transfecting of the pSVL constructs into COS-1 cells. Numbers in parenthesis represent fold increase in phosphohydrolase activity over the WT construct. Data represent the mean ± SEM. *p < 0.05, **p < 0.005.