Fig. 1.

Overview of experimental procedures. After seeding of 1 × 10⁵ cells/ml, the cells were exposed to 8% stretch stimuli at a stretching frequency of 0.1 Hz for 24 hr (1 stretch stimulation per 10-sec period). Cells cultured for 24 hr without stretching (0% stretch stimulus) were used as controls. After the stretch stimulation, the localization of intracellular Ca²⁺ and target gene expression in the stretched cells were measured.