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. 2019 Feb 28;81(4):586–592. doi: 10.1292/jvms.18-0372

Table 1. The sequences and modification of the LAMP and PCR primers used in this study.

Method Assay Primar name Sequence Ref.
DNA strip 18S rRNA of Sarcocystis spp. (SarcoR) F3 TGAAAACCTTGGCCGATAGG This study
B3 CCTTGTTACGACTTCTCCTTCCTC
FIP [tag]-GTAATCGGCGCAAGCTGCTGACGGTAATCTTTTGAGTATGCATCG
BIP [bi]-TGTACACACCGCCCGTCGCTCTTTCCATTCCGCACACGTTG
LF CTACTAGGCATTCCTCGTTGAAGAT
LB CTACCGATTGAGTGTTCCGGTGA

stx1 F3 GCGATTTATCTGCATCCCCGTATGTCTGGTGACAGTAGCTAT [4]
B3 GGAACCTCACTGACGCAGTCCTTCAGCTGTCACAGTAACA
FIP ACTGATCCCTGCAACACG
BIP TGTGGCAAGAGCGATGTT
LF ACAACAGCGGTTACATTGT
LB GATCATCCAGTGTTGTACGAA

stx2 F3 GGCGTCATCGTATACACAGGAGCGCTTCAGGCAGATACAG
B3 AGACGTGGACCTCACTCTGAAACTCTGACACCATCCTCTC
FIP CAGACAGTGCCTGACGAA
BIP GGCGAATCAGCAATGTGC
LF GCATCCAGAGCAGTTCTG
LB CAGTATAACGGCCACAGTC

Conventional PCR 18S rRNA of Sarcocystis spp. (SarcoR) F GGATAACCGTGGTAATTCTATG [18]
R TCCTATGTCTGGACCTGGTGAG

stx1 mStx1_F GGATAATTTGTTTGCAGTTGATGTC [17]
stx1 mStx1_R CAAATCCTGTCACATATAAATTATTTCGT
stx2 mStx2_F GGGCAGTTATTTTGCTGTGGA
stx2 mStx2_R GAAAGTATTTGTTGCCGTATTAACG