DNA strip |
18S rRNA of Sarcocystis spp.
(SarcoR) |
F3 |
TGAAAACCTTGGCCGATAGG |
This study |
B3 |
CCTTGTTACGACTTCTCCTTCCTC |
FIP |
[tag]-GTAATCGGCGCAAGCTGCTGACGGTAATCTTTTGAGTATGCATCG |
BIP |
[bi]-TGTACACACCGCCCGTCGCTCTTTCCATTCCGCACACGTTG |
LF |
CTACTAGGCATTCCTCGTTGAAGAT |
LB |
CTACCGATTGAGTGTTCCGGTGA |
|
stx1 |
F3 |
GCGATTTATCTGCATCCCCGTATGTCTGGTGACAGTAGCTAT |
[4] |
B3 |
GGAACCTCACTGACGCAGTCCTTCAGCTGTCACAGTAACA |
FIP |
ACTGATCCCTGCAACACG |
BIP |
TGTGGCAAGAGCGATGTT |
LF |
ACAACAGCGGTTACATTGT |
LB |
GATCATCCAGTGTTGTACGAA |
|
stx2 |
F3 |
GGCGTCATCGTATACACAGGAGCGCTTCAGGCAGATACAG |
B3 |
AGACGTGGACCTCACTCTGAAACTCTGACACCATCCTCTC |
FIP |
CAGACAGTGCCTGACGAA |
BIP |
GGCGAATCAGCAATGTGC |
LF |
GCATCCAGAGCAGTTCTG |
LB |
CAGTATAACGGCCACAGTC |
|
Conventional PCR |
18S rRNA of Sarcocystis spp.
(SarcoR) |
F |
GGATAACCGTGGTAATTCTATG |
[18] |
R |
TCCTATGTCTGGACCTGGTGAG |
|
stx1 |
mStx1_F |
GGATAATTTGTTTGCAGTTGATGTC |
[17] |
stx1 |
mStx1_R |
CAAATCCTGTCACATATAAATTATTTCGT |
stx2 |
mStx2_F |
GGGCAGTTATTTTGCTGTGGA |
stx2 |
mStx2_R |
GAAAGTATTTGTTGCCGTATTAACG |