Table 3. Comparison of conventional PCR and DNA strip for detection of Sarcocyctis spp., Toxoplasma gondii and STEC in Japanese sika deer.
Sample No. |
Sex | Sarcocystis spp. | Toxoplasma gondii | STEC | ||
---|---|---|---|---|---|---|
Conventional PCR | DNA strip | ELISA | Conventional PCR | DNA strip | ||
1 | M | - | - | NT | - | - |
2 | F | - | - | NT | - | - |
3 | M | - | - | NT | - | - |
4 | M | - | - | NT | - | - |
5 | F | - | - | NT | - | - |
6 | M | - | + | NT | - | - |
7 | M | - | - | NT | - | - |
8 | F | + | + | NT | - | - |
9 | F | + | + | - | - | - |
10 | F | + | + | - | - | - |
11 | F | - | - | - | - | - |
12 | M | - | - | - | - | - |
13 | F | + | + | - | - | - |
14 | F | + | + | NT | - | - |
15 | F | + | + | - | - | - |
16 | M | + | + | - | - | - |
17 | F | + | + | - | - | - |
18 | F | + | + | - | - | - |
19 | M | + | + | - | - | - |
20 | F | + | + | - | - | - |
21 | M | + | + | - | - | - |
22 | M | + | + | - | - | - |
23 | M | + | + | - | - | - |
24 | M | - | + | - | - | - |
25 | M | - | + | - | - | - |
26 | F | + | + | - | - | - |
27 | F | + | + | - | - | - |
28 | F | + | + | - | - | - |
29 | F | + | + | - | - | - |
30 | F | + | + | - | - | - |
31 | F | - | + | - | - | - |
32 | F | + | + | - | - | - |
33 | F | - | - | - | - | - |
34 | F | + | + | - | - | - |
35 | F | + | + | - | - | - |
36 | M | + | + | - | - | - |
37 | F | + | + | - | - | - |
38 | F | + | + | - | - | - |
39 | M | + | + | - | - | - |
40 | F | - | + | - | - | - |
41 | unknown | + | + | - | - | - |
42 | M | + | + | - | - | - |
43 | F | - | - | - | - | - |
44 | M | + | + | - | - | - |
45 | unknown | + | + | - | - | - |
46 | unknown | + | + | NT | - | - |
47 | unknown | + | + | NT | - | - |
Total number of positive sample | 32 | 37 | 0 | 0 | 0 |
NT, not tested; +, positive; -, negative.