Figure 3.

siRNA-Mediated Silencing of SEC61A1 Abolished the Effects of ES1 and ES24 on Ca²⁺ Homeostasis

(A and B) The time courses of TG responses in cytosolic Ca²⁺ illustrate the loss of effects of ES1 (A) and ES24 (B) on Ca²⁺ leakage after silencing SEC61A1 with two different siRNAs (SEC61A1#4 and SEC61A15). Experiments with control siRNA (scrRNA) and DMSO controls (mock) are superimposed for comparison. Application times are indicated by vertical lines (mock/ES1, mock/ES24, 1 μM TG).

(C and D) Relative amplitudes of cytosolic TG responses (post-TG Δ[Ca²⁺]_cyt) were used to quantify the effects of ES1 (C) and ES24 (D) in the experiments illustrated in (A and B), respectively.

(E and F) Relative contents of Sec61α protein in cells treated with SEC61A1 siRNAs are given as percentage of the Sec61α content in cells treated with scrRNA for the experiments with ES1 (E) and ES24 (F).

Data obtained in six different silencing experiments are presented as means ± SEM. n.s., not significant; ***p < 0.001. N = 94–222 cells per experimental setting. See also Figure S3.