Figure 5.
ES24 Reduces Cell Viability in a Ca2+-Dependent Manner
(A) HEK, INS-1, HeLa, and NALM-6 cells were exposed for 24 h to various ES compounds (ES) and cell viability was measured as the percentage of living cells. While ES1 was cytotoxic to all cell lines, ESR35 was not cytotoxic at all and ES24 displayed mild effects on some cell lines.
(B) Comparison of cell viabilities for 24-h treatments with 20 μM ES24 and 1 μM TG. A strong Ca2+-dependent component in cytotoxicity of ES24 is suggested by the linear correlation (black line) of the cell viabilities measured in the individual cell lines after 24 h treatment with 20 μM ES24 and 1 μM TG.
Color and symbol coding for cell lines and substances are shown below panels. Cell viability was measured per duplicate in three independent experiments, normalized to DMSO controls and is presented as means ± SD.