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. 2019 Apr 25;9:6527. doi: 10.1038/s41598-019-42944-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mean log-transformed levels of gonadal mRNA of male (mis) and female (foxl2 and cyp19a1a) biomarkers normalized to the reference gene ef-1α and plotted relative to each other from years 2014 to 2017. Shown above are dimorphic gonadal expression patterns for each marker (A) mean log mis as a function of mean log foxl2 (B) mean log mis as a function of mean log cyp19a1a. Females are indicated by circles and males are indicated by squares. Samples from the Pamlico region are shown as circles/squares with an “x”, the Neuse region as black circles/squares, and the South region as white circles/squares.